Cell Migration

Cell Migration

I have significant experience in the processing, visualisation and analysis of the movement of red blood cells in dorsal skin chambers and white blood cells, especially neutrophils and macrophages moving in zebrafish embryos. This experience has been also used in tracking small crustaceans and even cars.

Research in this area is closely related to Cancer and Microcirculation; please explore the other research pages and the publications below.

Team members:

Carlos Fernando Ramos-Lopez

Research Assistant
Alumni, University of Sussex

Jose Alonso Solis-Lemus

PhD Student
Alumni, City, University of London

Konstantinos Blazakis

PhD Student
Alumni, University of Sussex

Collaborators:

Abhir Bhalerao

Reader in Computer Science, University of Warwick

Brian Stramer

Randall Centre for Cell & Molecular Biophysics, King’s College London

Chryso Kanthou

School of Medicine, University of Sheffield

Graham Lieschke

Australian Regenerative Medicine Institute, Monash University, Australia

Greg Slabaugh

Director of the Digital Environment Research Institute, Queen Mary, University of London

Gillian Tozer

School of Medicine, University of Sheffield

Robert Knight

Dentistry, Oral & Craniofacial Sciences, King’s College London

Stephen A Renshaw

Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield

Publications:

Activation of hypoxia-inducible factor-1α (Hif-1α) delays inflammation resolution by reducing neutrophil apoptosis and reverse migration in a zebrafish inflammation model Philip M. Elks, Fredericus J. van Eeden, Giles Dixon, Xingang Wang, Constantino Carlos Reyes-Aldasoro, Philip W. Ingham, Moira K. B. Whyte, Sarah R. Walmsley, Stephen A. Renshaw Published in Blood, July 2011 (see publication) Research themes: Cell Migration Type: Paper

Pulses of Class I PI3kinase activity identify the release and recapture of prey from neutrophil phagosomes Clare Felicia Muir, Constantino Carlos Reyes-Aldasoro, Felix Ellett, Tomasz Prajsnar, Yin Xin Ho, Audrey Bernut, Catherine A Loynes, Stone Elworthy, Kieran A Bowden, Ashley J Cadby, Lynne Prince, Jason S King, Alison M Condliffe, Stephen Renshaw" Published in bioRxiv, February 2024 (see publication) Research themes: Cell Migration Type: Pre-Print

Cell Tracking Profiler - a user-driven analysis framework for evaluating 4D live-cell imaging data Claire Mitchell, Lauryanne Caroff, andJose Alonso Solis-Lemus, Constantino Carlos Reyes-Aldasoro, Alessandra Vigilante, Fiona Warburton, Fabrice de Chaumont, Alexandre Dufour, Stephane Dallongeville, Jean-Christophe Olivo-Marin, Robert Knight Published in Journal of Cell Science, November 2020 (see publication) Research themes: Cell Migration Type: Paper

Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection José Alonso Solís-Lemus, Besaiz J. Sánchez-Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro Published in Journal of Imaging, May 2020 (see publication) Research themes: Cell Migration Type: Paper

β-glucan-dependent shuttling of conidia from neutrophils to macrophages occurs during fungal infection establishment Vahid Pazhakh, Felix Ellett, Ben A. Croker, Joanne A. O'Donnell, Luke Pase, Keith E. Schulze, R. Stefan Greulich, Aakash Gupta, Constantino Carlos Reyes-Aldasoro, Alex Andrianopoulos, Graham J. Lieschke Published in PLoS Biology, September 2019 (see publication) Research themes: Cell Migration Type: Paper

Macrosight A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab® José Alonso Solís-Lemus, Brian Stramer, Greg Slabaugh, Constantino Carlos Reyes-Aldasoro Published in Journal of Imaging, January 2019 (see publication) Research themes: Cell Migration Type: Paper

Shape analysis and tracking of migrating macrophages José Alonso Solís-Lemus, Brian Stramer, Greg Slabaugh, Constantino Carlos Reyes-Aldasoro Published in ISBI, April 2018 (see publication) Research themes: Cell Migration Type: Conference

An objective comparison of cell-tracking algorithms Ulman, Vladimír and Maška, Martin and Magnusson, Klas E. G. and Ronneberger, Olaf and Haubold, Carsten and Harder, Nathalie and Matula, Pavel and Matula, Petr and Svoboda, David and Radojevic, Miroslav and Smal, Ihor and Rohr, Karl and Jaldén, Joakim and Blau, Helen M. and Dzyubachyk, Oleh and Lelieveldt, Boudewijn and Xiao, Pengdong and Li, Yuexiang and Cho, Siu-Yeung and Dufour, Alexandre C. and Jean-Christophe Olivo-Marin, Constantino C. Reyes-Aldasoro, Jose A. Solis-Lemus, Robert Bensch, and Brox, Thomas and Stegmaier, Johannes and Mikut, Ralf and Wolf, Steffen and Hamprecht, Fred A. and Esteves, Tiago and Quelhas, Pedro and Demirel, Ömer and Malmström, Lars and Jug, Florian and Tomancak, Pavel and Meijering, Erik and Muñoz-Barrutia, Arrate and Kozubek, Michal and Ortiz-de-Solorzano, Carlos Published in Nature Methods, December 2017 (see publication) Research themes: Cell Migration Type: Paper

Whole cell tracking through the optimal control of geometric evolution laws Konstantinos N. Blazakis, Anotida Madzvamuse, Constantino Carlos Reyes-Aldasoro, Vanessa Styles, Chandrasekhar Venkataraman Published in Journal of Computational Physics, September 2015 (see publication) Research themes: Cell Migration Type: Paper

Automatic segmentation of focal adhesions from mouse embryonic fibroblasts Constantino Carlos Reyes-Aldasoro, Muruj Barri, Majid Hafezparast Published in ISBI, April 2015 (see publication) Research themes: Cell Migration, Medical Imaging Type: Conference

Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics Chryso Kanthou, Gabi U. Dachs, Diane V. Lefley, Andrew J. Steele, Claudia Coralli-Foxon, Sheila Harris, Olga Greco, Sofia A. Dos Santos, Constantino C. Reyes-Aldasoro, William R. English, Gillian M. Tozer Published in PLoS ONE, January 2014 (see publication) Research themes: Cancer, Cell Migration Type: Paper

Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response Sofia de Oliveira, Constantino C. Reyes-Aldasoro, Sergio Candel, Stephen A. Renshaw, Victoriano Mulero, Angelo Calado Published in Journal of Immunology, April 2013 (see publication) Research themes: Cell Migration Type: Paper

PhagoSight an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model K.M. Henry, Luke Pase, C.F. Ramos-Lopez, Graham J. Lieschke, Stephen A. Renshaw, Constantino Carlos Reyes-Aldasoro Published in PLoS ONE, January 2013 (see publication) Research themes: Cell Migration Type: Paper

Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish Luke Pase, Judith E. Layton, Christine Wittmann, Felix Ellett, Cameron J. Nowell, Constantino Carlos Reyes-Aldasoro, Sony Varma, Kelly L. Rogers, Chris J. Hall, M. Cristina Keightley, Philip S. Crosier, Clemens Grabher, Joan K. Heath, Stephen A. Renshaw, Graham J. Lieschke Published in Current Biology, October 2012 (see publication) Research themes: Cell Migration Type: Paper

Local affine texture tracking for serial registration of zebrafish images Abhir Bhalerao, Luke Pase, Graham Lieschke, Stephen Renshaw, Constantino Carlos Reyes-Aldasoro Published in ISBI, May 2012 (see publication) Research themes: Cell Migration, Medical Imaging, Texture Type: Conference

The neutrophil’s eye-view inference and visualisation of the chemoattractant field driving cell chemotaxis in vivo Visakan Kadirkamanathan, Sean R. Anderson, Stephen A. Billings, Xiliang Zhang, Geoffrey R. Holmes, Constantino C. Reyes-Aldasoro, Philip M. Elks, Stephen A. Renshaw, Published in PLoS ONE, January 2012 (see publication) Research themes: Cell Migration Type: Paper

Microflow of fluorescently labelled red blood cells in tumours expressing single isoforms of VEGF and their response to vascular targeting agents Simon Akerman, Constantino Carlos Reyes-Aldasoro, Matthew Fisher, Katie L. Pettyjohn, Meit A. Björndahl, Helen Evans, Gillian M. Tozer Published in Medical Engineering & Physics, September 2011 (see publication) Research themes: Cancer, Cell Migration, Circulation Type: Paper

Application of intravital microscopy in studies of tumor microcirculation Sarah Jane Lunt, Colin Gray, Constantino Carlos Reyes-Aldasoro, Stephen J. Matcher, Gillian M. Tozer Published in Journal of Biomedical Optics, February 2010 (see publication) Research themes: Cancer, Cell Migration, Circulation Type: Paper

Analysis of immune cell function using in vivo cell shape analysis and tracking C. C. Reyes-Aldasoro, Y Zhao, D Coca, S. A. Billings, V. Kadirkamanathan, G. M. Tozer, S. A. Renshaw Published in 4th IAPR International Conference on Pattern Recognition in Bioinformatics, September 2009 (see publication) Research themes: Cell Migration Type: Conference

Measuring cellular migration with image processing C. C. Reyes-Aldasoro, D. Biram, G. M. Tozer, C. Kanthou Published in Electronics Letters, June 2008 (see publication) Research themes: Cancer, Cell Migration Type: Paper

Estimation of apparent tumor vascular permeability from multiphoton fluorescence microscopic images of P22 rat sarcomas in vivo Constantino Carlos Reyes-Aldasoro, Ian Wilson, Vivien E. Prise, Paul R. Barber, M. Ameer-Beg, Borivoj Vojnovic, Vincent J. Cunningham, Gillian M. Tozer Published in Microcirculation, January 2008 (see publication) Research themes: Cancer, Cell Migration, Circulation Type: Paper